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Alternative splicing is a mechanism used to create different isoforms of the same protein so that several protein isoforms can be created from a limited repertoire of genes. Splice sequences are found at the 5′ and 3′ tails of introns marked with the dinucleotide GU at its 5′ end and AG at the 3′ end. Exons are segments containing the encoding sequence for protein synthesis, while introns are intervening segments of nucleotides that will be excised from the primary RNA transcript (pre-mRNA) in a process called splicing. The transcribed RNA contains multiple segments called exons and introns. The consensus sequence TATTAA (called TATA box) is located 25–35 bases upstream of the initiation site, followed by a poly-G/C consensus sequence located 32–38 bases upstream. The initiation complex recognizes the promoter region in the DNA as a signal for the transcription starting point. The RNA polymerases are aided in the transcription function by a series of proteins called transcription factors, which have several functions mainly to facilitate the binding of RNA polymerase II to the promoter sequence, forming the transcription initiation complex. RNA polymerase III synthesizes 5S RNA, and all transfer RNA (tRNA) is located in the nucleoplasm. Only some proteins have a quaternary structure as well. Each protein at least contains a primary, secondary, and tertiary structure. The quaternary structure also influences the three-dimensional shape of the protein and is formed through the side-chain interactions between two or more polypeptides. The three-dimensional shape of a protein, its tertiary structure, is determined by the interactions of side chains from the polypeptide backbone. These hydrogen bonds create alpha-helix and beta-pleated sheets of the secondary structure. The secondary structure contains regions of amino acid chains that are stabilized by hydrogen bonds from the polypeptide backbone. Only some proteins have a quaternary structure as well. The primary structure is comprised of a linear chain of amino acids. Every protein at least contains a primary, secondary, and tertiary structure. A protein can be identified based on each level of its structure. They carry out a wide variety of organism functions, including DNA replication, transporting molecules, catalyzing metabolic reactions, and providing structural support to cells. ![]() Proteins are polypeptide structures consisting of one or more long chains of amino acid residues. ![]() strikes "against terrorist targets outside areas of active hostilities" (i.e., outside Afghanistan, Iraq, and Syria) with between 2,372 and 2,581 combatant deaths and between 64 and 116 non-combatant deaths, i.e., a civilian casualty rate of 2.63–4.30%. The Office of the Director of National Intelligence reported that: Office of the Director of National Intelligence drone strikes for Yemen, Afghanistan and Somalia, the BIJ figures include both drone strikes and other actions, including airstrikes, missile attacks, and ground operations. For Pakistan, the BIJ figures below cover only U.S. strikes from January 2004 through February 2020. The Bureau of Investigative Journalism (BIJ) reported the following figures for U.S. strikes in Somalia: between 1,389 and 1,696 militants, between 34 and 121 civilians, and between 56 and 69 unknowns. Collectively, between 1,479 and 1,886 people have been killed in the U.S. Of the 263, the majority (202) occurred under the Trump administration. counter-terrorism airstrikes, drone strikes, and ground raids from 2003 to 2021. In Somalia, there were a total of 263 U.S.strikes killed between 238 and 298 people, of whom between 227 and 277 were combatants, and between 11 and 21 were civilians. carried out 550 strikes (from all methods, not just drones), almost all of them in Operation Odyssey Lightning in 2016. ![]() In Libya, from the end of the 2011 UN-approved military intervention through March 31, 2020, there were 4,500 air, drone, and artillery strikes in Libya, carried out by many warring Libyan factions and their foreign supporters including the American, Russian, United Arab Emirates, Turkish, and Egyptian governments.Over those 18 years, there were 374 reported strikes in Yemen (with the peak being in 2012), which killed a total of between 1,378 and 1,775 people of the total deaths, between 115 and 149 were civilians. drone strike in Yemen as of the report's publication was May 17, 2020. drone strike in Yemen was Novem(killing al-Qaeda operative Qaed Salim Sinan al-Harethi), and the most recent U.S. Over those 14 years, there were 413 reported strikes in Pakistan (with the peak being in 2010), which killed a total of between 2,366 and 3,702 people of the total deaths, between 245 and 303 were civilians. drone strike as of the report's publication was in Pakistan on July 4, 2018. drone strike in Pakistan was June 19, 2004, and the most recent U.S. Civilian casualties as a percentage of overall deaths were highest in Yemen and lowest in Somalia. drone strikes in Pakistan, Yemen, and Somalia from 2009–2016, with a broadly similar rate from 2017–2019. Taken together, independent estimates from the non-governmental organizations New America and the Bureau of Investigative Journalism suggest that civilians made up between 7.27% to 15.47% of deaths in U.S. The "estimates of civilian casualties are hampered methodologically and practically" civilian casualty estimates "are largely compiled by interpreting news reports relying on anonymous officials or accounts from local media, whose credibility may vary." Total numbers Independent estimates Determining precise counts of the total number killed, as well as the number of non-combatant civilians killed, is impossible and tracking of strikes and estimates of casualties are compiled by a number of organizations, such as the Long War Journal (Pakistan and Yemen), the New America Foundation (Pakistan, Yemen, Somalia, and Libya), and the London-based Bureau of Investigative Journalism (Yemen, Somalia, and Pakistan). Drone strikes are part of a targeted killing campaign against militants. Since the September 11 attacks, the United States has carried out drone strikes in Pakistan, Yemen, Somalia, Afghanistan, Iraq and Libya. drone strikes A United States Air Force drone Overview of civilian casualties from U.S. ![]() 'The Saga' at Main Plaza - 9pm, 9:30pm & 10pm every Tuesday, Friday, Saturday & Sunday. Hop onto our open-top red bus and explore the Cape Town with over 30 stops, and taking you to over 50 of Cape. Take in panoramic views from an open-top double-decker minibus, and disembark at any of the 12 stops to check out one of the main attractions or simply go for a walk. Address: 111 Alamo Plaza Street, 78205 (next to Kilwins Ice-Cream). Make the most of your time in the principality with a hop-on hop-off bus tour in Monaco. Lobos tour + Cabo Giro tour Hop on Hop Off Up to 1h30 duration tours 48h. What's FREE at City Sightseeing's new Tours & Tickets Shop?įor our customers we provide FREE water, FREE WiFi and FREE charging stations. Hop on and hop off as much as you like at any of the bus stops on the route and see all the best sights and attractions your chosen town or city has to offer. With the hop-on hop-off system, you can begin and step out whenever you want. Children 4 years old and younger ride FREE. LEGOLAND Discovery Center and Shops at River Center MallĬhildren are 5-11 years old.This Copenhagen hop-on hop-off tour ensures you get the most out of your time in the Danish capital as it provides a helpful. Market Square El Mercado and Centro des Artes Museum Copenhagen Hop-on Hop-Off Bus with Boat Option. ![]() City Sightseeing Amsterdam Hop-On, Hop-Off, Cruise + Rijksmuseum Ticket. City Sightseeing Amsterdam: Hop-On Hop-Off & Canal Cruise. Of course you are free to get off and explore at any of our 19 stops. City Sightseeing Amsterdam: Hop-On, Hop-Off Bus. We get to each stop every 20 minutes, from 8.40am to 5.30pm. With the option of indoor or open-air seating, our Hop-On Hop-Off Bangkok sightseeing bus tour is a great way to start your trip in the city of angels. The story of San Antonio starts here - your tour begins in Alamo Plaza, immediately giving you the fantastic 'double decker' view of the beautiful Alamo - and then explores the River Walk district and historic neighborhoods.Įxperience this old south Texas city as you head north over the River Walk to The Pearl, Spanish Governor's Palace, Cathedral, Theater District, Tower of the Americas, Mexican Market Square, King William Historic District and much much more. Explore with Hop on Hop off' - Your Global Adventure Awaits. ![]() To avoid too many alerts, we used activity zones, which excluded sections of the viewing field from motion detection. About CVR, Do-It-Yourself Monitoring, & Pro Monitoring And since it’s hardwired, you’ll never have to take down the Nest Cam Floodlight to recharge. If you buy the Nest Cam Floodlight, it comes with complete mounting accessories, including wire connectors, mounting plates, and even screws. A strong Wi-Fi signal and a fully-charged battery is all it needs.Īnd while the other outdoor camera, the Nest Cam Floodlight, requires hardwiring, it’s relatively easier to install than other smart light-equipped cameras. You don’t need to plug it in or connect it to a recorder. As the name suggests, this small but sturdy camera comes with a battery, making installation a breeze. The best outdoor camera in Nest’s lineup is the Nest Cam (battery). ![]() It offers products like video doorbells and smart speakers, but their flagship home products belong to the Google Nest Cam line of security cameras. Nest, a Google-owned brand, is a leading smart home and home security brand. ![]() With some models, you can even connect the DVR to the internet and playback stored videos on your smartphone. With Lorex, outdoor cameras are typically hooked up to a DVR or NVR – essentially a computer box with a 1 TB or more hard drive. And without continuous recording, you risk missing those important events. That works too, but important moments happen sometimes without triggering a camera’s motion sensor. Most outdoor cameras are configured to start recording when they detect motion. That feature may not be favorable for an indoor camera – you don’t want eyes recording your every move inside your house – but for an outdoor camera, continuous recording adds a lot of value. Lorex’s outdoor cameras are special because unlike other popular options, they can record continuously. Mounted Lorex Camera Continuous Recording Weatherproof Lorex cameras with 4K resolution started at just $159. Resolution on some Lorex camera models was“ultra-high” at 4K, whereas resolution on others was merely “high” at 1080p. For instance, the 1080 HD Outdoor Wireless Security Camera ($149) had a 115-foot range in the dark. They were virtually vandal proof and had long depths of field with color night vision. Compared with leading home security cams, Lorex Wi-Fi and IP cameras were even more durable and high-performing. Lorex Technology had the best selection of industrial-quality outdoor security cameras. Which one stood out as the outdoor security champion of 2023? Keep reading to find out! Furthermore, the best security cameras made crisp recordings in any light most had infrared night vision. ![]() They also prevented it! With built-in features such as motion-activated floodlights and sirens, these cameras easily scared trespassers away. Our hands-on experience with scores of outdoor security cameras taught us that these devices were not only valuable for recording crime. They can either be wired or wireless the former provides maximum reliability while the latter offers installation flexibility. These devices, which can often withstand rain and extreme temperature, offer traditional security camera features like night vision, two-way talk, and HD resolution. The Role Outdoor Cameras Play in Home Security
However, when only using two of these (any. The problem is, when I do all three of these things, after a few loops the Arduino seems to crash and stop responding. My program connects to a server to which it relays information from 2 sensors and then gets a reading back. Note that this task could have been programmed without "while". Hey, I'm working on a project using an Arduino Uno, and I'm fairly new to working with them so please bear with me. I have a problem while connecting Arduino Uno R3 using ESP8266 ESP-01 module with Blynk. The previous one initialized "startMs" only when loop() started the first time. Theres probably a way around this using a while loop to keep calling. If You try to execute it the second time, nothing will be displayed. As long as it is true (for 10 sec.), the next instruction will execute, printing milliseconds.Ĭan You find a subtle error in the code? Hint: it will run only once. Now, "while": in the brackets is a logical expression (evaluates either true or false). For example:įirst line declares a variable that will be retained between successive executions of loop(), named "startMs", which immediately stores number of milliseconds since microcontroller power-up. # after initializing serial, an arduino may need a bit of time to reset Root.after(10, readSerial) # check serial again soon SerBuffer += "\n" # add the newline to the buffer # get the buffer from outside of this function ![]() I thought I approached the correct way but when the function is called it keep running so appearently Im doing something wrong. #Serial.readline seems unreliable at times tooĬ = ser.read() # attempt to read a character from Serial Hello, Im trying to run a while loop for 200ms. Log = Text ( root, width=30, height=30, takefocus=0) # make a text box to put the serial output ![]() Ser = Serial(serialPort, baudRate, timeout=0, writeTimeout=0) #ensure non-blocking This problem can be avoided with the timeout=0 option when enitializing the Serial object, which will cause it to return nothing unless something is already waiting in the Serial object's buffer. For example, a Serial.readline() won't print anything until there is a whole line to return, which in some cases might be never! Even using the after() and update() methods will still not allow the UI to be updated in this case, since the function never ends. Normally, the Serial.read() and Serial.readline() will hold up the whole program until it has enough information to give. arduino serial.Serial (port'COM4', baudrate115200, timeoutNone, dsrdtrTrue) This is the when solve with this method. ![]() However, the real issue is making sure that reading from serial is non-blocking. Check if there are options in python to setup the port without forcing DTR it should be something in the line of DTRenable or so. Both methods achieve basically the same goal of updating the GUI. Alternatively, you could write your own infinite loop, and call root. If you are running your own infinite loop anywhere in the code, the GUI will freeze up. It needs the things in there to run every now and then in order to make the interface respond to interactions. mainloop() method, it is running its own while loop. Keep in mind that when TkInter gets to the root. after(milliseconds) method to run a non-blocking version of read in the tkinter main loop. The process cannot update buttons or react to input because it is busy waiting for the serial to say something. However, waiting for input from pySerial's Serial object is blocking, which means that it will prevent your GUI from being responsive. ![]() To use Python as a graphical interface for an Arduino powered robot, programmatically read the USB with the pySerial library. Cook, stirring occasionally, until onion is softened and starting to brown, 5–7 minutes. Add 1 medium onion, chopped, and season with kosher salt. unsalted butter in a large ovenproof skillet with a lid over medium-high heat. ![]() Slice stems crosswise ½" thick cut crowns into 2" florets. Trim 2 large heads of broccoli (about 1½ lb.), then separate stems from crowns. cayenne pepper in a medium bowl to combine. cans condensed cream of mushroom soup, 1 cup sharp cheddar, grated, zest of 1 lemon, 1 tsp. ![]() And if you’re looking for more broccoli recipes, we’ve got those too. For more easy recipes, check out these Thanksgiving vegetable side ideas. Trying to map out your holiday menu? You can bake this casserole a few hours ahead of time and then reheat it at 350° to warm through and re-crisp the topping, about 10 minutes. If you don’t have an ovenproof skillet, use any large, lidded skillet to cook the onions in melted butter and steam the broccoli, then toss the broccoli with the mushroom soup mixture and transfer to a baking dish before topping with the shredded cheese and crushed cracker crumbs. While most casserole recipes are typically baked in a casserole dish, this one calls for an ovenproof lidded skillet so you can sauté the onions, steam the broccoli (no need to blanch!), and bake off the casserole all in one pan. All told, it’s bound to be a favorite among picky eaters who may otherwise avoid their veggies. Instead of breadcrumbs or panko, we swear by Ritz crackers for the crispy, crunchy topping, but any good buttery cracker will do. In addition to fresh broccoli florets, we slice up the delicious broccoli stems too, so nothing goes to waste-don’t be tempted to swap it out for frozen broccoli, which could turn to mush in the oven. ![]() The cream of mushroom soup creates a rich base, while sharp cheddar cheese, lemon zest, garlic powder, lots of black pepper, and a touch of cayenne add flavor and intrigue to the creamy sauce. As a side dish, it’s the perfect addition to any potluck, dinner party, or all-out Thanksgiving spread (move over green bean casserole) though we won’t tell if you serve it up as a weeknight main dish instead. Think of this broccoli casserole recipe as a cousin of another beloved comfort food: broccoli-cheddar soup. ![]() Another 3 to 6 inches will add to snow metro totals, according to Lisa. So far, Denver has seen 40.5 inches of snow for the 2022-2023 season. In elevations above 9,000 feet heavy snow is expected, between 12 and 24 inches, the NWS said. Saturday: Air Force Academy, CO 13 inches at 6:27 a.m. The winter storm warning is now in effect in West Jackson and West Grand Counties. Up to 17,000 were without power on the morning of May 12 in northern Colorado, according to Xcel Energy in Aurora, Colorado. The following Colorado snow totals have been reported by the National Weather Service for May 21, 2022, as of 8:10 a.m. Colorado Snow Forecast, Colorado 24 hr New Snow Accumulation Analysis. The weight of this heavy, wet snow damaged trees in Cedar City, Utah on Sunday. Incidentally, Wyoming's capital city has also had a pair of seven-inch-plus snow days in early June (eight inches on Jun. They've had a pair of 10-inch-plus snow days later in the calendar (and May 16, 1943), but slightly less than Sunday's total of 10.5 inches. Weve now received about 40 of our seasonal snowfall with our. 1.1 inches officially at Denver Int'l Airportĭating to 1883, Sunday was the heaviest calendar-day snow so late in the season in Cheyenne, by a narrow margin. Denvers official total at Denver International Airport was 7.1 inches, which is the highest total from any storm so far this season.Snow totals around Denver, Colorado and the Front Range from the most recent February. 4.0 inches in Denver's Stapleton neighborhood (former airport site) A winter storm moved through the Denver metro area on Wednesday, leaving behind several inches of fresh snow.12.0 inches officially in Cheyenne, Wyoming (up to 13.3 inches in metro) Exactly one month until the summer solstice, Colorado is waking up to a winter wonderland.29 inches near Four Corners in north-central Colorado.30.1 inches near Pinecliffe, Colorado (Jefferson Co.43 inches estimated at Divide Peak in the Sierra Madre range of Wyoming.Here are some notable snow totals from Winter Storm Zephyr: 16.Winter Storm Zephyr dropped heavy snow in the Rocky Mountains and even into parts of the Front Range urban corridor May 10-13, 2014. The first to open in 2021 was Wolf Creek, on Oct. MONDAY NIGHT: We stay mostly dry with a spot storm possible through the evening hours around the Pikes Peak Region. Arapahoe Basin was the first to open last year, on Oct. Sunday, here are some Colorado snow totals for the winter storm, according to the National Weather Service: Cripple Creek, 20 inches. Monday’s snow came 38 days before the 10-year average date for Colorado’s first ski area opening of the season, which is Oct. Overnight low temperatures in Summit County this week will be in the 30s, according to the National Weather Service. Elsewhere in the Tenmile Range there was snow on Crystal Peak and Pacific Peak, a pair of prominent thirteeners that are visible from Copper Mountain resort. ![]() (Provided by Copper Mountain Resort)īreckenridge ski resort received snow across its five peaks, according to spokeswoman Sara Lococo, as did Bald Mountain, a 13,700-foot peak just east of town. The peaks of the Tenmile Range received a dusting of snow Monday, as seen from Copper Mountain. You can view the snow accumulation, snow depth, and snowfall for your recent winter storms as well as nearby snow reports from weather stations across the. ![]() Trail Ridge Road in Rocky Mountain National Park was closed for about two hours Monday morning due to snow and ice. The following Colorado snow totals have been reported by the National Weather Service for Dec. Friday: Air Force Academy, CO 2.5 inches at 7 a.m. Several high peaks in Colorado received noticeable dustings of snow overnight including Pikes Peak, the peaks of the Tenmile Range in Summit County, the Arapahoe Basin ski area and Capitol Peak, a fourteener near Aspen. The following Colorado snow totals have been reported by the National Weather Service for Nov. ![]() Friday, October 6th 2023 Home Page Close Menu ![]() It immediatedly left customers facing technical failures and ‘significant disruption’ to TSB’s branch, telephone, online and mobile banking services. The programme involved a migration to a new IT platform in April 2018, from a system operated by its former owner, Lloyds Banking Group, to one designed by its current owner, the Spanish bank Sabadell. The Financial Conduct Authority (FCA) and the Prudential Regulation Authority (PRA) have fined TSB Bank £48,650,000 for “operational risk management and governance failures”, including management of outsourcing risks, relating to the bank’s IT upgrade programme. ![]() TSB bank has been fined £48.65m over a notorious botched IT migration which left customers locked out of their bank accounts for days back in 2018. Good morning, and welcome to our rolling coverage of business, the financial markets and the world economy. Not all telephone banking services are available 24/7.08.40 CET Introduction: TSB fined over IT migration meltdown TSB Bank plc is covered by the Financial Services Compensation Scheme and the Financial Ombudsman Service.Ĭalls may be monitored and recorded in case we need to check we have carried out your instructions correctly and to help us improve our quality of service. Read more about our lending commitments to you (PDF, 56.8KB). ![]() TSB adheres to The Standards of Lending Practice which are monitored and enforced by the Lending Standards Board. SC95237.Īuthorised by the Prudential Regulation Authority and regulated by the Financial Conduct Authority and the Prudential Regulation Authority under registration number 191240. Registered office: Henry Duncan House, 120 George Street, Edinburgh EH2 4LH. Android, Google Pay, and the Google Logo are trademarks of Google LLC. registered in the US and other countries. For example, if you have a monthly standing order and don’t have enough money to pay it three months in a row, we’ll cancel it after this happens.Īpple, the Apple logo, Apple Pay, Apple Watch and iPhone are trademarks of Apple Inc. If you don’t have enough money in your account to pay a standing order three times in a row, we’ll cancel it. If you do deposit enough money into your account to meet the payment that you wish to make, the payment will be made and you won’t incur a Returned Item Fee. The money needs to be immediately available to use, so you could transfer money from another account you have with us by Mobile Banking, Internet Banking, Telephone Banking or by paying in cash over the counter in a branch. Shortly after midnight on the due date or next working dayįor standing orders and future dated payments, we’ll also try a third time shortly after midnight the net working day after we first tried to make the payment.Our Retry Periods mean that if you don’t have enough money in your account to allow us to pay Direct Debits, cheques, standing orders and future dated payments, we’ll give you extra time to put money into your account to allow us to make these payments.įor Direct Debits and cheques, we’ll try to take the payment: The money needs to be immediately available to use, so you could transfer money from another account you have with us by Mobile Banking, Internet Banking, Telephone Banking, paying in cash over the counter in branch or transferring the funds from another provider. ![]() Our Grace Period means that you have until 10pm (UK time) to pay enough money into your account to avoid any Overdraft interest that we may charge that day. This engine endows the RAV4 with reasonable straight-line acceleration, though the engine sounds rather gruff when spinning at higher revs. Front-wheel drive is the default setup, and all-wheel drive is optionally available. The least powerful setup is a 203-hp 2.5-liter four-cylinder engine that mates to an eight-speed automatic gearbox. The 2023 Toyota RAV4 offers three different power source options. Fortunately, we've done some of the work for you by comparing these two models' key specifications. Determining which one makes the most sense for your needs and budget, though, may require a little bit of extra research. Its more compact size, however, means the Corolla Cross sacrifices interior space relative to the larger RAV4.ĭespite their differences, these two Toyota models are sure to be cross-shopped by many consumers looking for a small, relatively affordable SUV. Measuring in at 175.6 inches long, the Corolla Cross is smaller than today's RAV4 but still notably larger than the first-gen four-door RAV4.Įven so, the Corolla Cross drives smaller than its size suggests, making it reasonably easy to navigate this subcompact SUV through crowded city streets. Those looking for a Toyota SUV closer in size to that of the original RAV4 may find themselves better served by the subcompact Corolla Cross. With an overall length of 180.9 inches, the 2023 RAV4 casts a shadow nearly 20 inches longer than its forebear from 1996 (the first model year that Toyota sold the RAV4 on our shores). ![]() The Toyota RAV4 is no longer the diminutive SUV it once was. |
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